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LulzBot FRESH™ Bio Printer [certified Bio] KT-PR0054NA
LulzBot FRESH™ Bio Printer [certified Bio] KT-PR0054NA
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The LulzBot Bio is a FRESH™ certified bioprinter ready to print bioinks, unmodified collagen, and other soft materials for pharmaceutical/cosmetic testing, tissue engineering, regenerative medicine, and so much more. You may be asking yourself what FRESH means and how it relates to bioprinting.
What is FRESH?
FRESH stands for Freeform Reversible Embedding of Suspended Hydrogels. This method of printing allows the Bio printer to suspend materials into a thermo-reversible viscous gel temporarily. Once a print is completed the LulzBot Bio’s heated bed can heat the gel to reverse the material’s viscous properties, turning it to a liquid and freeing the print. Having a viscous gel as a support material for bioprinting allows materials, such as unmodified collagen, alginate, or other soft materials, to be printed into delicate and difficult organic geometries that standard AM printers are incapable of producing.
The best and most reliable form of thermo-reversible hydrogel for the FRESH process is LifeSupport® from FluidForm. Composed of sterile and dried gelatin microparticles. LifeSupport® is quicker and has optimal properties that have higher reproducibility than other methods of FRESH. LifeSupport® can be purchased from LulzBot.
Early iterations of bioprinters forced materials to be modified and printed in the air, compromising their physiological relevance. The FRESH process allows materials to be printed unmodified and even benefit from the chemistry used when preparing the gel support; ionic, pH, enzymatic. This can help guide a tissue to develop properly.
FRESH is the only method where a bioprinter can print the most physiologically relevant cells and materials. For proper tissue function to happen there must first be proper tissue structure. Bioprinters need to be able to assemble a structure the same as any other biological organism. With the FRESH process this is achievable by having complete control over spatial alignment as well as printing soft deformable materials at a high resolution.
Before the LulzBot Bio, traditional forms of bioprinting failed at creating a high enough level of cell density to achieve functionality. Since FRESH eliminates the need to print materials in air, bioinks can focus on having more cell density. It is also very critical in bioprinting that a printer can create biopharma tissue models and also implantable tissue. Meaning you need a printer and a material that can scale to variable sizes. FRESH has those desirable capabilities, only being limited to the size of container the viscous gel is placed in
Bioprinting by LulzBot
LulzBot BIO for Life Support
Features
Real Materials, Real Innovation
The LulzBot Bio is optimized for Freeform Reversible Embedding of Suspended Hydrogels (FRESH™) printing. Out of the box, users can print real, unmodified collagen, alginate, and other soft materials. This brings researchers closer than ever before to recreating human physiology for in vitro testing and regenerative medicine. Combined with an ultra-precise syringe pump extruder, the Bio is ready for a wide variety of bioprinting applications, from creating multi-scale vasculature to fully functional heart tissue and more.
Heated Bed
The LulzBot Bio’s variable temperature heated bed allows full control of environmental conditions during printing, ensuring the integrity of the print from start to removal.
High-Detail Bioprinting
The syringe pump extruder design allows for precise control of material extrusion, enabling printing of high-detail objects in a wide range of materials with varying viscosities.
Material Options
The LulzBot Bio features an open materials system which includes ready-to-print profiles for alginate and unmodified collagen. With a configurable syringe pump system and extrusion settings, the Bio is easily adjustable for new materials and processes.
Designed for the Lab
The small footprint and large build volume easily fits within laminar flow cabinets or on lab countertops. The rugged design can easily be sterilized for printing cells using ethyl alcohol and UV lights.
Easy-To-Use Interface
The Bio features a 4.5" glove-friendly touch screen and SD card slot for easy operation without the need to be tethered to a computer.
Open Source, Open Possibilities
Innovation in research demands flexibility. the Bio's FRESH™ Certified and Open Hardware Design gives researchers the keys to innovate, allowing machine capabilities to be easily adjusted for new materials and processes. the Bio's adaptable configuration can be modified to extrude:
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Other components of the extracellular matrix (hyaluronic acid, fibrin)
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Photo-curable resins
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Thermosets
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Silicon and epoxies
Key Specifications and Materials
- Technology: Fluid Deposition Fabrication (FDF)
- Tool Head: Syringe Pump Extruder
- Min. Layer Resolution: 0.05 mm (50 μm)
- Min. Positive Feature Size: 0.08 mm (80 µm)
- XYZ Positional Accuracy: 10, 10, 5 μm
- Materials: FRESH™ certified. Ready to print profiles for alginate and unmodified collagen.
- Certifications: ETL/UL, CSA, FRESH, OSHWA
|
Printing
| Print Technology | FRESH™ Certified |
| Print Volume Dimensions | 160mm x 160mm x 89mm* (6.30in x 6.30in x 3.5in*) |
| Print Volume | 2,278.4cm³ (138.9in³) |
| Ambient Operating Temperature | From 5°C to 40°C (41°F to 104°F) |
| Operating Footprint | 76.2cm x 63.5cm x 53.3cm (30in x 25in x 21in) |
| Maximum Travel Speed | 200 mm/sec |
| Connectivity | USB Serial or SD Card |
| Interface | 4.5" Glove-friendly touch-screen |
| Included Tool Head |
Syringe Pump Extruder (compatible with 2.5mL, 5mL, and 10mL) |
| Provided Syringe | Hamilton 2.5 mL Gastight Syringe Model 1002 TLL, PTFE Luer Lock |
| Needle Diameter | 50, 25 gauge and 50, 30 gauge dispensing needles included |
| Print Surface | Heated Borosilicate Glass |
| Maximum Print Bed Temperature | Up to 110°C (248°F) |
| Print Bed Heat Up Time | 23°C to 60°C (73°F to 140°F) in 97 seconds |
| Minimum Layer Resolution | 0.05mm (50 μm) |
| Minimum Positive Feature Size | 0.08 mm (80 µm)** |
| Calculated XYZ positional resolution | 10, 10, <5 micron |
Materials
| Supported Materials | FRESH Certified open material system with ready-to-print profiles for collagen and sodium alginate |
Additional Items Included
| Comprehensive Tool Kit | Includes: Tool Kit Bag, 2.5 mL Gastight Glass Syringe, 25 gauge dispensing needles, 30 gauge dispensing needles, Hypodermic Leur Lock, Cole-Palmer Female Leur , Cole-Palmer Male Leur, 35mm Plastic Petri Dish, 50 mL KIMAX crystallizing dish, Celltreat Petri Dish, Spoon Scoopula, Specimen Cup w/ Lid, Transfer Pipette, Ball Hex Key Wrench Set, Power Cord, USB Cord, SD Card, Syringe Thumb Screws, Syringe Base and Clamps |
| Comprehensive Documentation | Includes: Quick Start Guide, Quality Assurance Record, and more. |
| First 3D Print Material | Includes Sodium Alginate, LifeSupport Gel, and everything you need to complete your first print. |
Physical Dimensions
| Boxed Unit Weight | 12kg (26.5lbs) |
| Unit Weight | 9kg (19.85lbs) |
| Boxed Dimensions | 55.8cm x 43.2cm x 48.3cm (22in x 17in x 19in) |
| Dimensions with Syringe Pump Extruder at Z max | 45.7cm x 33.9cm x 49.53cm (18in x 13in x 19.5in) |
| Average Operating Dimensions | 76.2cm x 63.5cm x 53.3cm (30in x 25in x 21in) |
Software
| Operating System Compatibility | GNU/Linux, Mac, Windows |
| Recommended Software | Cura LulzBot Edition Version 3.2 or newer |
| Firmware | Marlin |
| Supported File Types | .stl, .obj, .g, .gcode, .x3d, .3mf |
Electrical
| Power Requirements | 100VAC-240VAC, 50Hz-60Hz, 3.2A max |
| Output | 24VDC |
| Power Supply | Auto-switching MEAN WELL RSP-500-24 |
| Power Cable | IEC cord, C13 to region-specific connector |
Safety and Compliance
| Certifications | ETL/UL, CSA, FRESH, OSHWA |
| Warranty | Includes one-year warranty and access to technical support five days a week. Optional one, two, or three-year extended warranties available. |
| Country of Origin | Made in USA |
LulzBot BIO Operations Guide
Quick Start Guide
LulzBot BIO Quick Start Guide
Request a Quote
For a quote contact us at support@precision3dworks.com
Or Call us at +1 (323)310-4428
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RETURN AND REFUND POLICY
Return and Refund Policy
Cancellations & Refunds: At Precision 3D Works we pride ourselves on having the best return policies in the industry. Cancel your order any time before it ships completely free of charge (exceptions apply such as custom orders, see below).
If you have questions, please send an email to support@precision3dworks.com or call us at +1(213) 698-8700
Cancellations (Before Order Ships)
If you need to cancel an order, please contact us as soon as possible so we can refund you in full before your order leaves the warehouse. Reach agents during business hours at +1(213) 698-8700, the chat in the bottom right, or email support@precision3dworks.com any time.
Cancellations of Custom Orders
Custom or made-to-order products cannot be canceled or refunded as these products are put into production specifically for your order.
These units are custom-made to your specification or created once you order. Once you place your order they start building your unit. Your warranty will guarantee you a fully functional and operational product. If it is not clear whether your order is custom or made to order, please message or call us.
All sales are final for Custom made and Made-to-order products.
Refunds and Returns.
If you need to exchange your product, you may do so. You, as the customer, are responsible for any shipping costs throughout any returns and exchange processes.
Due to drastically increasing LTL/freight shipping costs, any outright returns will incur a 25% cancellation fee + return shipping costs. If you are replacing the product with something else, we may be able to help you cover some of the costs depending on the product.
Returns Accepted Within 30 Days
Please note that we will only accept returns within 30 days from the date you receive the product. Beyond this period, we will not process returns.
Shipping Times
We promise to do our best to get your order to you as soon as possible. We provide estimated shipping times on the product pages. All orders are dispatched within 1-2 business days and delivered within 5-7 business days.
Order delays have proven to be unavoidable and outside of Precision 3D Works control. While this might be frustrating, we ask you to please be patient and understanding with us in these tough times. We thank you for your business and appreciate your patience.
Please Read the Following
When you are making a purchase from Precision 3D Works, you are acknowledging the following of the Exchange Agreement:
- I understand I am to inspect the package upon delivery, and notate and take pictures if there is any damage and provide it to Precision 3D Works within 24 hours of the product being delivered.
- I understand that I am responsible for the cost of return shipping and the cost of a new product shipping in the case of a refund or exchange.
- I understand that products need to be returned unopened and unused. An additional restocking fee may occur.
- I understand that if my order has left the warehouse, I am unable to receive a full refund.
- I understand that if I return my order once it has shipped, I will be responsible for paying any return shipping fees and restocking fees, which vary by product, but are usually around 25%.
Damages
Please inspect the packaging of your item(s) when they arrive, if you notice any damage, you should make note of it when signing for delivery. If your item(s) arrive damaged please send photos of the damage, box, and SKU, along with a brief description of the damage to support@precision3dworks.com and we will process an insurance claim on your behalf. Photos and videos must be sent for freight damage within 24 hours of arrival or the freight damage claim will not be valid. Any punctures or visible signs of exterior damage must be reported within 24 hours.
Warranty
Warranties vary depending upon the manufacturer - see individual product pages for details. Warranty damages occur over time and from use. If an item was damaged upon arrival, and not reported within 30 days, that is not a warranty claim.
Returns
The Precision 3D Works default is that customers will be responsible for all return shipping charges or re-consignment fees caused by customer error, unless otherwise stated on the product page.
All of our products have guaranteed warranty policies - meaning that you will 100% get a fully functional, operating product that you paid for without any extra expenses incurred on your end - please see individual product pages for details.
BOGO Promotions
In the event of a BOGO promotion, both items must be returned for a full refund. If one of the two items is returned, you will get a replacement of that item or store credit for the value of that item when purchased on its own. If your order has shipped, you (the buyer) will also be responsible for actual return shipping charges and depending on the manufacturer, potentially a restocking fee. Refunds will only be issued to the original credit card that you used when placing your order.
All customers agree that they have read, understand, and agree to the terms and conditions above.
Individual items will have varying refund and return policies depending on the manufacturer. Please see individual product pages for details or contact support@precision3dworks.com
Chargebacks
Our team of agents is here to assist you and resolve any issues. We have purposefully made our policies as fair as possible, and we believe they are the top in the industry. We are on your team. We are immediately responsive and will do all that we can to help you out and provide the best resolution.
Given this, any customer that files a fraudulent chargeback will be held criminally liable for theft. If you have not received a product, or have an issue with a product that you did receive, please contact us, and we will help you resolve your issue. Please do not file chargebacks for issues that we can resolve together. Thank you for shopping with us!
Contact support@precision3dworks.com for any questions.
BY USING THE SITE, YOU AGREE TO THESE TERMS OF USE; IF YOU DO NOT AGREE, DO NOT USE THE SITE.